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中文名称

Adenosine Deaminase(ADA)Calibrator(2 mL Vial)(阿登西汀脱氨酶(ADA)Calibrator(2 mL Vial))

英文名字
Adenosine Deaminase (ADA) Calibrator (2 mL Vial)
供应商
Aviva Systems Biology
产品货号
aviva-GWB-BQK2A7-1U
产品报价
¥询价/1 x 1 mL
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该Adenosine Deaminase (ADA) Calibrator (2 mL Vial)的背景资料请参考该产品的说明书
产品描述
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Product NameAdenovirus IgM - ELISA.
# of Samples1 x 96 Assays.
Intended UseThe Adenovirus IgM ELISA is intended for the qualitative determination of IgM class antibodies against Adenovirus in human serum or plasma (citrate).
IntroductionAdenoviruses are double-stranded DNA viruses of about 70-90 nm lacking an envelope. The capsid contains 252 capsomeres and shows icosahedral symmetry. The capsomeres consist of hexons, pentons and fiberprotein trimers which are responsible for the induction of group- and type-specific antibodies.
For the first time adenoviruses were isolated in 1953 from tonsils and adenoid tissue by Rowe. More than 80 adenoviruses are known at present. 47 out of them are pathogenic for men. They cause several diseases of different organic systems, mainly eyes, pharynx, respiratory and gastrointestinal system.
Adenovirus infections are common and frequent. Most infections appear during childhood. They pass of latently so that the virus can still be detected in tonsils after two years. It is excrete via saliva and faeces. Gate to body are mouth, nasal pharynx and conjunctiva of the eye. Most infections pass off without symptoms. Around 5 % of all coughs and sneezes of children are caused by adenoviruses. Epidemics may occur in populations crowded together, for example acute respiratory disease in military groups, pharyngoconjunctival fever in swimming pools, and epidemic keratoconjunctivitis in medical facilities. Infection of hospitals and swimming pools gratify special demands on hygienics. The presence of virus resp. infection may be identified by
Cell culture: cytopathogenic effect (CPE)
PCR
Serology: complement fixation (CF), neutralization (N) and hemagglutination-inhibition (HAI); Detection of antibodies and the hexon antigen by ELISA.
Principles of the assayThe qualitative immunoenzymatic determination of IgM-class antibodies against Adenovirus is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.
Microtiter strip wells are precoated with Adenovirus antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgM conjugate is added. This conjugate binds to the captured Adenovirus-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Adenovirus specific IgM antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
Storage and StabilityThe reagents are stable up to the expiry date stated on the label when stored at 2-8C.
ReferencesAdrian,T.,J.Sassinek,R.Wiegand:Genome type analysis of 480 isolates of adenovirus type 1,2 and 5.Arch Virol.112 (1990) 235-248Donelli,G.,F.Superti,A.Tinari et al.: Viral childhood diarrhoe in Rome: a diagnostic and epidemiological study.Microbiologica 16 (1993) 215-225Hierholzer,J.C.: Adenovirus in the immunocompromised host. Clin.Microbiol.Rev.5 (1992) 262-274Schmitz,H.,R.Wiegand,W.Heinrich: Worlwide epidemiology of human adenovirus infectionsw.Am.J.Epid.117 (1983) 455-466Wiegand,R.:Adenovirus.In:Brandis,H.,W.Köhler,H.J.Eggers,G.Pulverer (ed.):Med. Mikrobiologie 1994.Gustav Fischer Verlag Stuttgart,Jena,New York (1994) 767-769
产品形式
该Adenosine Deaminase (ADA) Calibrator (2 mL Vial)的产品形式请参考该产品的说明书
保存建议
该Adenosine Deaminase (ADA) Calibrator (2 mL Vial)的保存建议请参考该产品的说明书
其他
Aviva Systems Biology总部位于加利福尼亚州圣迭戈,在中国北京设有办公室,专注于为研究需求提供多克隆和单克隆抗体、ELISA试剂盒、蛋白质和定制服务。Aviva Systems Biology生产了24,000种经过验证的多克隆抗体,并提供近20,000种ELISA试剂盒,定制实验室服务包括蛋白表达和纯化、抗体开发,以及ELISA的开发、验证和生产。Aviva Systems Biology为与独特物种和靶标相关的研究提供独特工具,研究领域包括转录因子、癌症、心血管、细胞生物学、DNA损伤和修复、表观遗传学、信号转导、细胞分化、干细胞生物学等等。
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