Biotinylated ubiquitin has utility as a detectable alternative to radio-iodinated ubiquitin for studying ubiquitination in vitro. It is readily activated by ubiquitin E1, is a competent substrate for known ubiquitin E2 / E3 enzymes (thioester bond formation) and can be conjugated to ubiquitin target proteins (isopeptide bond formation). Use of biotinylated ubiquitin also enables affinity purification of modified substrates using avidin/streptavidin.
Contains multiple biotinylated ubiquitin species ([Bt]1-5) with (Bt)2-4 as the predominant species as observed by mass spectrometric analysis
