NEDD8 E2 conjugating enzyme. Recombinant protein expressed with N-terminal His-tag
Cullin-RING ligases (CRLs) comprise the largest subfamily of E3 ubiquitin ligases. In humans, six cullins (CUL1, -2, -3, -4A, -4B, and -5), two RBX-family RING proteins (RBX1 and -2), and hundreds of substrate receptors assemble into distinct CRLs that mediate ubiquitination of thousands of targets to regulate a vast array of cellular processes. CRL function is regulated by attachment of the ubiquitin-like protein (UBL) NEDD8 to a conserved lysine in a cullin
