- 中文名称
Tissue Inhibitor of Metalloproteinases 2 (TIMP2) BioAssay ELISA Kit
- 英文名字
- Tissue Inhibitor of Metalloproteinases 2 (TIMP2) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- T5585-75
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- 产品描述
- The TIMP-2 immunoassay kit utilizes antibodies immobilized on a bead matrix, in combination with enzyme-labeled antibodies, directed against different antigenic sites on the same TIMP-2 molecule. Upon addition of a TIMP-2-containing specimen, the result is a TIMP-2 molecule being sandwiched between the solid phase and enzyme labeled antibodies. After removing unbound enzyme-labeled antibody, the bead containing the sandwich is incubated with enzyme substrate and TMBZ, resulting in the development of color. The activity of peroxidase enzyme is proportional to the amount of antigen, TIMP-2, so that TIMP-2 concentration in specimens can be determined from a standard curve. The TIMP-2 immunoassay kit is useful for the determination of TIMP-2 levels in fresh serum and conditioned medium of cells. This system does not recognize TIMP-2 complexed with proMMP-2. While the immunoreactivities of TIMP-2 complexed with active MMP-1, MMP-2 or MMP-9 are increased by approximately 300%, 450%, and 300%, respectively, compared with that of the free form of TIMP-2, no change is observed when TIMP-2 is complexed with active MMP-3. Contents of this kit are sufficient for assay of 100 samples, including standard curve. Testing of samples in duplicate or triplicate is strongly recommended. This kit is intended for research use only; not for diagnostic or therapeutic applications. Sensitivity: 20ng/ml Range of Detection: 20ng/ml – 320ng/mlKit Components:1. Anti-TIMP-2 Coated Beads–One (1) bottle containing 100 anti-TIMP-2 coated polystyrene beads.2. Concentrated Enzyme Labeled Antibody Solution–One (1) 1ml bottle containing TIMP-2 antibody.3. Color Reagent– One (1) 1ml bottle 3,3',5,5'-Tetramethylbenzidine (TMBZ)4. Buffer for Color Reagent: 40ml Acetate buffer, pH 5.5.5. Substrate Solution–One (1) 15ml bottle 0.02% Hydrogen Peroxide.6. Stop Solution–Two (2) bottles, sufficient to make 100ml 1.3 N Sulfuric acid per bottle.7. Buffer Reagent (Lyophilized)–One (1) 50ml bottle BSA in sodium phosphate buffer.8. TIMP-2 Standard (Lyophilized)–One (1) bottle, sufficient to make 1ml Human TIMP-2 Standard.9. Concentrated Wash Solution–Two bottles, sufficient to make 1L Sodium phosphate buffer, pH 7.0, per bottle.Storage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Tissue Inhibitor of Metalloproteinases 2 (TIMP2) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Tissue Inhibitor of Metalloproteinases 2 (TIMP2) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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