- 中文名称
Thyroid Stimulating Hormone, Human, Neonatal (TSH) BioAssay ELISA Kit
- 英文名字
- Thyroid Stimulating Hormone, Human, Neonatal (TSH) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- T5400-01A
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Thyroid-stimulating hormone (TSH) is secreted by the anterior lobe of the pituitary gland and induces the production and release thyroid hormones thyroxin (T4) and triiodothryronine (T3). These thyroid hormones exert a negative feedback on the pituitary. The release of TSH is regulated by TSH-releasing hormone (TRH) produced in the hypothalamus. When there are high circulating levels of thyroid hormone in the blood, less TRH is released by the hypothalamus, so less TSH is secreted by the pituitary. The normal concentration of TSH in the blood is extremely low, but it is essential for maintenance of normal thyroid function. The determination of serum or plasma levels of TSH is recognised as a sensitive method in the diagnosis of primary and secondary hypothyroidism. Primary Congenital Hypothyroidism (CH) occurs in 1 out of every 3,000 to 7,000 infants and is caused by athyroidism and hypoplasia. If infants are screened for this disorder during their first month, then irreversible mental retardation can be prevented through early diagnosis and proper treatment. The state of infant's thyroid can be determined by a T4 and TSH combination-screening program. This is the most effective method for the clinician because secondary hypothyroidism may be missed by some TSH screenings and T4 screenings may miss minimal hyperthyroidism. Before starting therapy, a confirmation test should be performed if an infant is thought to be suffering from marginal or borderline hypothyroidism. These determinations should be performed using serum T3, T4, and TSH. Due to infant age, weight, prematurity, and demographic variation concentrations of TSH and T4 have been shown to have some variation. Thus each laboratory must establish its own normal and cut-off values. This kit uses a method of collecting blood spot samples on S&S #903 filter paper and ELISA techniques. This kit can quantitatively determine TSH level in neonates sensitively, accurately, safely and reliably. It is an important and practical tool to determine thyroids state of neonates, thus making it possible to prevent against infant mental retardation.The Neonatal TSH quantitative enzyme immunoassay described as a solid phase enzyme linked immunosorbent assay (ELISA). Monoclonal antibodies, specific to TSH, have been bound to the surface of each microplate well. During the course of the assay, a blood sample (collected on filter paper) is added to the microplate wells with Sample Buffer and incubated overnight. After washing the microplate to remove the filter paper and unbound component of the sample, a standardized preparation of horseradish peroxidase-conjugated monoclonal antibody specific for TSH • unit is added to each well and incubated. The TSH, if present in the sample, will bind to the antibody on the coated well and will form an Antibody-TSH-Antibody-HRP “sandwich”. The microplate wells are thoroughly washed to remove unbound conjugate. Next, a TMB (3,3', 5,5' tetramthyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a 15-minute incubation period. Only those wells that contain TSH and enzyme-conjugated antibody will exhibit a change in color. The enzymesubstrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm. In order to measure the concentration of TSH in the test sample, this neonatal TSH ELISA Kit includes calibration standards and controls. The calibration standards and controls are assayed at the same time as the test samples and allow for the operator to produce a standard curve of optical density versus TSH uIU/ml, serum. Therefore, by comparing the optical density of the samples to this standard curve, the concentration of the TSH in the test samples is then determined.Sensitivity: <0.25uIU/mlRange: 0.25-150uIU/mlKit Components:1. Reaction Microplate, 1x96 wells 2. Enzyme Conjugate, 1x12ml 3. TSH Standard Calibrator Card, 1x1 sheet4. TSH Control Card, 1x1 sheet 5. Sample Collection Card, 1x90 sample6. Substrate A, 1x10ml 7. Substrate B, 1x10ml 8. Stop Solution, 1x12ml 9. Sample Buffer 1x12ml 11. Wash Buffer (20X), 1x60mlStorage and Stability:Store components at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Thyroid Stimulating Hormone, Human, Neonatal (TSH) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Thyroid Stimulating Hormone, Human, Neonatal (TSH) BioAssay ELISA Kit产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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