- 中文名称
Thyroid Stimulating Hormone, Human, Low Range (TSH) BioAssay ELISA Kit
- 英文名字
- Thyroid Stimulating Hormone, Human, Low Range (TSH) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- T5400-01
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- This ultra-sensitive TSH enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for TSH. Standards or samples are then added to the microtiter plate wells and TSH if present, will bind to the antibody precoated on the wells. In order to quantitate the amount of TSH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TSH are added to each well to “sandwich” the TSH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, a TMB (3,3', 5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TSH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm.In order to measure the concentration of TSH in the sample this Ultra-sensitive Human TSH ELISA Kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density (O.D.) versus TSH concentration (uIU/ml). The concentration of TSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: <0.05uIU/mlRange: 0.05-12uIU/mlKit Components:1. Microtiter Plate, 1x96 wells2. Conjugate, 1x12ml3. Standard, 12uIU/ml, 1x1 vial4. Standard, 6 uIU/ml, 1x1 vial5. Standard, 2ulU/ml, 1x1 vial6. Standard, 0.8ulU/ml, 1x1 vial7. Standard, 0.2ulU/ml, 1x11 vial8. Standard, 0ulU/ml, 1x1 vial9. Substrate A, 1x10ml10. Substrate B, 1x10ml11. Stop Solution, 1x7ml, 2N Sulphuric Acid (H2SO4). Storage and Stability:Store components at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Thyroid Stimulating Hormone, Human, Low Range (TSH) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Thyroid Stimulating Hormone, Human, Low Range (TSH) BioAssay ELISA Kit产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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