- 中文名称
S6 Ribosomal Protein, Total, BioAssay ELISA Kit discontinued
- 英文名字
- S6 Ribosomal Protein, Total, BioAssay ELISA Kit discontinued
- 供应商
- USBiological
- 产品货号
- S0050-45
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions (2). This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors (2,3). The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, are located within a small 19 amino acid region in the S6 carboxy terminus (4,5).Total S6 Ribosomal Protein Sandwich ELISA Antibody Pair is being offered as an alternative to our Total S6 Ribosomal Protein Sandwich ELISA Kit. Capture and Detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. The S6 Ribosomal Protein Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added, followed by S6 Ribosomal Protein Detection Antibody and HRP-conjugated secondary antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of Total S6 Ribosomal protein.Kit Components: S6 Ribosomal Protein Capture Antibody (100X), 1x0.4ml S6 Ribosomal Protein Detection Antibody (100X), 1x0.4ml Anti-Rabbit IgG HRP-Linked Antibody (1000X), 1x0.04ml Reagents not supplied: Phosphate Buffered Saline (PBS-20X)Phosphate Buffered Saline with Tween -20 (PBST-20X) Cell Lysis Buffer (10X)TMB SubstrateSTOP SolutionBlocking Buffer- PBS+0.05% Tween-20, 1% BSA 96 Well MicroplatesMicroplate Reader Storage and Stability:S6 Ribosomal Protein Capture Antibody and S6 Ribosomal Protein Detection Antibody are stored at 4°C. Anti-Rabbit IgG-HRP Linked Antibody is stored at -20°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对S6 Ribosomal Protein, Total, BioAssay ELISA Kit discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到S6 Ribosomal Protein, Total, BioAssay ELISA Kit discontinued产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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