- 中文名称
绵羊来源多克隆抗体：Connexin-40（254-270）/Cx40/254 antisera：whole serum
- 英文名字
- Sheep antibody to Connexin-40 (254-270)/Cx40/254 antisera: whole serum
- 供应商
- Biosensis
- 产品货号
- S-063-100
- 产品报价
- ￥询价/100uL
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- Biosensis品牌专注于神经科学领域的抗体和试剂的开发，在神经科学领域属于全球领航者，被广泛用于阿尔茨海默氏症（AD）、帕金森氏症（PD）和肌萎缩侧索硬化（ALS）疾病，以及自噬和代谢应激障碍的研究。武汉艾美捷科技作为Biosensis品牌中国区域总代理，提供Biosensis品牌特色的组织染色方案：FJC退化神经元染色试剂盒、病理髓鞘染色试剂盒、淀粉样斑块染色试剂等。武汉艾美捷科技拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队，可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 应用类型
- WB; IF, Frozen;
Immunohistochemistry: Antibody detects Cxn 40 in rat tissues and arterial endothelial cells. The authors report that the density of Cx40 plaques was significantly greater in the caudal artery (CA) than in the thoracic aorta (ThA), whereas no such difference was seen for Cx37 and Cx43. Expression of Cx40 was absent from the media of both thoracic and caudal artery tissues (see Rummery, NM et al 2002 for more staining specifics).
Published Method: Unfixed 10 µm thick sections cryosections or lightly fixed (2% paraformaldehyde in 0.1 mol/L sodium phosphate buffer) whole mount sections have been tested, see Rummery, NM et al 2002). Pretreatments include pre-incubation for 30 minutes in a blocking solution of 2% bovine serum albumin (BSA), 0.2% Triton-X in PBS, followed by primary antibody incubation. Antibody was used at 1:100 to 1:250 for 1 hour in the original work but Biosensis recommends optimizing the conditions for the best results. Original detection was via Cy3- conjugated anti-goat immunoglobulins (Jackson Immunoresearch Laboratories Inc, PA, 1:100) in 0.01% Triton-X in PBS, but other secondary conditions should work as well once optimized. In the original work the specificity of each antibody was tested by incubation either without primary antibody or with primary antibody that had previously been pre-incubated for 1 hour at room temperature with 10-fold excess by weight of the peptide against which the antibody was raised. (Adapted from Rummery, NM et al 2002).
Western Blot: Antibody is not recommended for western blots by Biosensis, however, it does react in westerns with Cxn 40 specific material. The authors report that the antibody develops numerous bands in westerns blots, only some of which are removed upon peptide treatment (see Rummery, NM et al 2002). The Cx40/254 antibody specifically recognized a band of 40 kDa from lung, caudal artery (CA), and thoracic aorta (ThA) but not liver (online Figure VIIA, +/- peptide). In the lung, however, a band at 45 kDa also appeared to be reduced with Cxn 40 peptide addition.
Published Method: Brain, heart, liver, lung, thoracic aorta and caudal arteries were removed from 5-6 week old Wistar rats and snap frozen in liquid nitrogen Tissues were ground under liquid nitrogen in a mortar and pestle and resuspended in 1mL of lysis buffer (1 mM NaHCO3 pH 7.05, 10 mM EDTA, 10 mM Iodoacetamide, 10 mM tetra-sodium pyrophosphate, 1 mM PMSF and 1 µg/mL each of antipain, aprotinin, pepstatin-A, chymostatin and leupeptin). Tissues were further disrupted by grinding in a polytron blender. Unbroken cells and large debris were removed by centrifugation at 1000 g for 5 minutes at 4oC, the supernatant was then removed and centrifuged at 3000 g for 5 minutes. The pellet was discarded, and the supernatant centrifuged at 20000 g for 15 minutes at 4°C. The supernatant was discarded, and the membrane-enriched pellet was resuspended in lysis buffer. Protein concentration was measured using the Bio-Rad protein assay kit. Membrane-bound connexins were subsequently solubilized by incubation in 2x SDS sample buffer (5% SDS, 125 mM Tris-Cl (pH 6.8), 20% glycerol, 2 mM _- mercaptoethanol, 0.1% (w/v) bromophenol blue) for 60 minutes at 37°C. Aliquots containing 5 µg of protein were separated by SDS-PAGE on 12% polyacrylamide gels and blotted onto PVDF membranes. Blots were probed with sheep antibodies against Cx40 (1:1000, Cx40/254) and detected via ECL using anti-Goat poly HRP secondary antibodies 1:4000, 1 hr. (adapted from Rummery, NM et al 2002).Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- 免疫原
- A synthetic peptide consisting of amino acids 254 to 270 of the C-terminus of rat Cx40 (Cx40/254) conjugated to diphtheria toxoid has been used as the immunogen.
- 来源宿主
- 绵羊
- 反应性
- reported that the Cx40/254 antibody did not cross react with 大鼠 Cx43 but may with Cx45 in Western blots only (Rummery, NM et al 2002).
- 保存建议
- 厂家建议常温运输。抗体溶解之后，建议分装保存于-20℃。当保存于4℃时，应添加适量的抗生素。按照1:1体积比添加最高纯度的甘油，可增加抗体的稳定性。请避免反复冻融。
- 其他
- Biosensis专注于神经科学领域的抗体和试剂的开发，特别是神经营养因子和神经营养因子受体。 近30年来，Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子，我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症（AD）、帕金森氏症（PD）和肌萎缩侧索硬化（ALS）疾病，以及自噬和代谢应激障碍，包括肥胖、代谢综合征的研究、神经免疫学和炎症。Biosensis的产品系列不仅包括持续增长的神经科学研究抗体系列，还包括200多种定量研究ELISAs试剂盒（1板或2板，自由选择），组织染色（退化神经元染色试剂盒、病理髓鞘染色试剂盒、淀粉样斑块染色试剂等）和细胞可视化试剂（染色）以及针对神经科学和细胞疾病研究的纯化的蛋白质。我们的抗体和试剂已被广泛应用于各种技术，包括蛋白质印迹，免疫组织化学，流式分析，共聚焦显微镜实时成像，生物抑制和细胞培养以及克隆。
- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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