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细胞分析试剂盒
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中文名称

RBP4, Mouse, BioAssay ELISA Kit (Retinol-binding Protein 4, Plasma Retinol-binding Protein, PRBP, RBP)

英文名字
RBP4, Mouse, BioAssay ELISA Kit (Retinol-binding Protein 4, Plasma Retinol-binding Protein, PRBP, RBP)
供应商
USBiological
产品货号
R1701-36Z
产品报价
¥1/100Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues. In obesity and type 2 diabetes, expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue. Adipocyte-specific deletion of Gluts leads to notable elevation of mouse RBP4 causing systemic insulin resistance, and that reduction of RBP4 improves insulin resistance. This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocyte-derived hormone. The RBP4 (mouse/rat) ELISA Kit is to be used for the in vitro quantitative determination of mouse or rat RBP4 in serum, urine and cell culture supernatant. This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG, peroxidase and TMB (3,3',5,5'-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of RBP4 in the samples. This ELISA is specific for the measurement of natural and recombinant mouse and rat RBP4. It does not cross-react with human RBP4, mouse adiponectin, mouse resistin, mouse Nampt, rat adiponectin, rat resistin, rat Nampt, mouse RELM-a, mouse RELM-b, human leptin, rat RELM-a, mouse 41BBL, mouse CD137, mouse Tim3, human TNF-a. The assay range is 0.188-12ng RBP4/ml and a detection limit of 60pg/ml (based on adding 2 standard deviations to the mean value of the (50) zero standards).Sample Type:Serum, urine, or cell culture supernatant.Intended Use:For the in vitro quantitative determination of mouse or rat RBP4 in serum, urine and cell culture supernatant.Sensitivity:60pg/ml Range:0.188-12ng RBP4/mlSpecificity:MammalianTest Principle:This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG, peroxidase and TMB (3,3',5,5'-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of RBP4 in the samples. This ELISA is specific for the measurement of natural and recombinant mouse and rat RBP4. It does not cross-react with human RBP4, mouse adiponectin, mouse resistin, mouse Nampt, rat adiponectin, rat resistin, rat Nampt, mouse RELM-a, mouse RELM-b, human leptin, rat RELM-a, mouse 41BBL, mouse CD137, mouse Tim3, human TNF-a.Kit Components:Pre-coated Microtiter Plate: 12x8 well stripsWash Buffer (10X): 1x50mlDiluent (5X):1x50mlDetection Antibody: 1x12mlDetector 100X (Hrp conjugated anti-IgG): 1x150ulMouse RBP4 Standard (lyophilized, 24ng): 1 vialMouse RBP4 QC Sample (lyophilized): 1 vialSubstrate Solution I (TMB): 1x6mlSubstrate Solution II (Peroxidase): 1x6mlStop Solution: 1x12mlPlate Sealers: 3 eachStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对RBP4, Mouse, BioAssay ELISA Kit (Retinol-binding Protein 4, Plasma Retinol-binding Protein, PRBP, RBP)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到RBP4, Mouse, BioAssay ELISA Kit (Retinol-binding Protein 4, Plasma Retinol-binding Protein, PRBP, RBP)产品后将其置于4°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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