- 中文名称
Resistin, Mouse, BioAssay ELISA Kit (Adipose Tissue-specific Secretory Factor, ADSF, Adipose-specific Cysteine-rich Secreted Protein A12-alpha, Cysteine-rich Secreted Protein FIZZ3, Fizz3, Retn)
- 英文名字
- Resistin, Mouse, BioAssay ELISA Kit (Adipose Tissue-specific Secretory Factor, ADSF, Adipose-specific Cysteine-rich Secreted Protein A12-alpha, Cysteine-rich Secreted Protein FIZZ3, Fizz3, Retn)
- 供应商
- USBiological
- 产品货号
- R1585-01W
- 产品报价
- ￥1/100Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Obesity is a well-known risk factor of type 2 diabetes mellitus and is strongly associated with insulin resistance. Resistin (also called FIZZ3/ADSF) is an adipocyte-derived peptide first identified during a search for targets of thiazolidinediones. Serum concentrations of resistin are markedly increased in obese mice and are decreased by treatment with thiazolidinediones. It was also found that administration of an anti-resistin antibody increases insulin-stimulated glucose uptake in obese mice and that treatment of normal mice with recombinant resistin impairs insulin action. Thus, resistin might link obesity with insulin resistance and diabetes in mice models. However, subsequent studies in rodent models have produced disparate findings on the role of resistin in obesity and insulin resistance. In humans, while the expression of resistin in human adipocytes is very low compared with that seen in rodents and does not differ between normal, insulin-resistant or type 2 diabetic individuals, a more recent study using a large size of case suggests that the plasma resistin levels are increased in type 2 diabetes. Therefore determination of the plasma resistin levels may be important for understanding onsets of metabolic diseases such as type 2 diabetes or obesity. This assay is a sandwich ELISA for quantitative determination of mouse resistin in biological fluids. A monoclonal antibody specific for resistin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, resistin is recognized by the addition of a biotinylated polyclonal antibody specific for resistin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of resistin in the samples. The assay range is 0.47-30ng/ml resistin/ml. The lowest level of resistin that can be detected by this assay is 100pg/ml.Sample Type:Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids.Intended Use:For the quantitative determination of mouse Resistin in biological fluids.Sensitivity:100pg/mlRange:0.47-30ng/ml Resistin/mlSpecificity:MammalianTest Principle:This assay is a sandwich ELISA for quantitative determination of mouse resistin in biological fluids. A monoclonal antibody specific for resistin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, resistin is recognized by the addition of a biotinylated polyclonal antibody specific for resistin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of resistin in the samples.Kit Components:Pre-coated Microtiter Plate: 12x8 well stripsWash Buffer (10X): 1x50mlDiluent (5X):1x50mlDetection Antibody: 1x12mlDetector 100X (HRP Labeled Streptavidin): 1x150ulMouse Resistin Standard (lyophilized): 16ngMouse Resistin QC Sample (lyophilized): 1 vialSubstrate Solution I (TMB): 1x6mlSubstrate Solution II (Peroxidase): 1x6mlStop Solution: 1x12mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Resistin, Mouse, BioAssay ELISA Kit (Adipose Tissue-specific Secretory Factor, ADSF, Adipose-specific Cysteine-rich Secreted Protein A12-alpha, Cysteine-rich Secreted Protein FIZZ3, Fizz3, Retn)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Resistin, Mouse, BioAssay ELISA Kit (Adipose Tissue-specific Secretory Factor, ADSF, Adipose-specific Cysteine-rich Secreted Protein A12-alpha, Cysteine-rich Secreted Protein FIZZ3, Fizz3, Retn)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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