WB; Western Blotting (WB). A dilution of 1:500-1:2,000 is recommended for WB. R-1776-100 has an epitope located in the extracellular domain portion of FGFR2. In tests using C6 cells, this antibody detects a 135 kDa band under reducing conditions Immunoprecipitation (IP). A concentration of 1:50 is recommended for IP in a lysis buffer that contains no more than 0.5% detergent as well as proteinase inhibitors. Detection can be via R-1776-100 in westerns following, IP enrichment however rabbit heavy chain (50-55~ kDa) and light chains (~23-25 kDa) will also be detected by the anti-rabbit used to detect R-1776-100 on westerns unless biotinylation of R-1776-100 is used. As always, Biosensis recommends optimal dilutions/concentrations should be determined by the end user for their specific experimental design.