WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human Protein kinase C alpha has a predicted length of 672 residues and MW of 77 kDa. A concentration of 0.5-1 µg/mL is recommended to detect the protein in paraffin embedded tissues. Antigen retrieval by boiling paraffin sections in 10 mM citrate buffer, pH 6.0 for 20 minutes is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide corresponding to a sequence of human PRKCA (153-182aa MDHTEKRGRIYLKAEVADEKLHVTVRDAKN), that differs from the related rat and mouse sequences by one amino acid. There is 100% alignment with various monkey species including Rhesus, Baboon, gorillas by BLAST as well as other mammals including horse and marmoset.
来源宿主
兔
反应性
人; predicted to react with 小鼠, 大鼠 due to sequence similarity.
