WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human ICAM1 (precursor) has a predicted length of 532 residues and a MW of 58 kDa. A concentration of 0.5-1 µg/mL is recommended to detect ICAM1 in paraffin embedded tissues. Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
E. coli-derived human ICAM1 recombinant protein (Position: L214-P532).Human ICAM1 shares 52% and 48% amino acid (aa) sequence identity with mouse and rat ICAM1, respectively.
