WB; Western Blotting (WB), IHC-P. A concentration of 0.1-0.5 µg/mL is recommended for WB. Human HIF-1 alpha (isoform 1) has a predicted length of 826 residues and a MW of 93 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect HIF1A in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required by boiling the formalin/paraffin sections in 10mM citrate buffer, pH6.0 for 20 mins. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide corresponding to a sequence at the N-terminus of human HIF-1-alpha (8-21aa NDKKKISSERRKEK), different from the related rat and mouse sequences by two amino acids.
