WB; WB: Western blot: reduced RIPA samples or membrane preparations (0.1-.5 µg/mL) dependent upon antigen load and antibody incubation times and detection substrate used.
IH: frozen or acetone fixed tissue: 0.5-1.0 ug.mL, permeabilization is necessary as epitope is internal IH(P): 1-2 µg/mL with HEIR treatment required.
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human CD44 cytoplasmic domain aa 728-742. The immunogen differs by only single amino acid between human, mouse and rat proteins.
