WB; Western Blotting (0.5 - 2 µg/mL). Cell lysates or membrane preparations prepared from isolated brain or spinal cord tissues are recommended. This antibody works on skim milk blocked membranes, however, best results are obtained with a equal mixture of 2.5% skim milk / 2.5% highly purified BSA as blocking reagent and antibody diluent. Immunohistochemistry (1 - 5 µg/mL). Antibody has been shown to work on PFA fixed, frozen sections. TBS is preferred for buffer preparation. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
Synthetic peptide immunogen, SNDDDSA[pS]PLHHIS
来源宿主
兔
反应性
人, 小鼠, other species not yet tested but predicted to react with 大鼠, 鸡 TrkB-pS478 based on amino acid homology at the site.