WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human Vascular endothelial growth factor (isoform VEGF206) has a predicted length of 232 residues and MW of 27 kDa. A concentration of 0.5-1 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide corresponding to a sequence at the N-terminus of human VEGFA(33-48aa VDIFQEYPDEIEYIFK), identical to the related mouse and rat sequences.
来源宿主
兔
反应性
人; 大鼠; expected to react with 小鼠 due to sequence homology