WB; IHC, Frozen; IH(P), Paraffin embedded; IF, Frozen; IF(P), Paraffin embedded; IHC, WB. This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 2000 depending on tissue and detection method. For western blotting a dilution range of 1: 1000 to 1: 4000 is recommended. This antibody stains prominently specific human airway (probably Clara cells) and alveolar type II cells and some tubular cells in the kidney. Other tissues have not yet been tested but the gene expression has also been reported in heart, pancreas and placenta. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (WTGEDSAEPNSDSAEWIRD) corresponding to the amino acids 18-37 of human extracellular superoxide dismutase conjugated to diphtheria toxin has been used as the immunogen.
