WB; WB and IHC. Suggested dilution of 1:5,000 is recommended for WB. DJ-1 is a soluble protein of 189 amino acids and detected with 23 kDa mobility by western blotting. The suggested dilution for IHC is 1:100. Detected astrocyte cytoplasmic labelling in human brain formaldehyde-treated tissue. Biosensis recommends that the optimal working dilution should be determined by the end user.
免疫原
A synthetic peptide (ASKRALVILAKGAEE-C) corresponding to human DJ-1【2-16] in the N-terminal domain conjugated via additional C-terminal Cys to Diphtheria toxoid.
