WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human Annexin I (precursor) has a predicted length of 346 residues and a MW of 39 kDa. A concentration of 0.5-2.0 µg/mL is recommended to detect Annexin I in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (MVSEFLKQAWFIENEEQEYVQT) corresponding to a region (3-24) from the N-terminus of human Annexin I.