WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. The predicted length of human Connexin-43 is 382 residues and MW of 43 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect Connexin-43 in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide corresponding to the amino acids 366-382 from the C-terminus of human Connexin-43.
