WB; IH,Frozen, IH(P), Paraffin embedded Western Blotting (WB) and Immunohistochemistry (IHC). A concentration of 1.0 µg/mL is recommended for WB. Human NOS (nNOS-1) has a predicted length of 1,434 residues and MW of 161 kDa. A concentration of 2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (IAFIEESKKDTDEVFSS) corresponding to a sequence (1418-1434 aa) from human Nitric Oxide Synthase 1.