WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human CXCR4 (isoform 1) has a predicted length of 352 residues and MW of 40 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in human formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval (Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins) is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (SGDYDSMKEPCFREEN) corresponding to a region (18-33) from human C-X-C Chemokine receptor 4. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
来源宿主
兔
反应性
人 (WB, IHC); 大鼠 (WB); predicted to react with 小鼠 due to sequence homology;
