WB; IH,Frozen, IH(P), Paraffin embedded Western Blotting (WB) and Immunohistochemistry (IHC). A concentration of 1.0 µg/mL is recommended for WB. Human Cullin-4B (isoform 1) has a predicted length of 895 residues and MW of 102 kDa. A concentration of 2.0 µg/mL is recommended for IHC in Zebra fish. Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (DRDYMERDKENPNQYNYIA) corresponding to a region (877-895) from the C-terminus of human Cullin-4B. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
