WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Zebra fish NTE has a predicted length of 1,348 residues and MW of 150 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Enzymatic antigen retrieval is required. Also the recommended concentration for formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (DEDKTVRQRKNVSRDPNADFSGVSDC) corresponding to a region (1323-1348) from the C-terminus of zebrafish Neuropathy target esterase. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
