WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Rat MRGPRX1 has a predicted length of 323 residues and MW of 37 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (TLNKTGHPSCRPILT) corresponding to a region (14-28) from rat Mas-related G-protein coupled receptor member X1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
