WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human p27 KIP 1 has a predicted length of 198 residues and MW of 22 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (NAGSVEQTPKKPGLR) corresponding to a region (180-194) from human p27 KIP 1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
来源宿主
兔
反应性
人; 大鼠; predicted to react with 小鼠 due to sequence homology;