WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human COX-2 (precursor) has a predicted length of 604 residues and MW of 69 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat activated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (NRGVCMSVGFDQYKCDCTRTGFY) corresponding to a region (28-50) from human Cyclooxygenase-2. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
来源宿主
兔
反应性
人; 大鼠; predicted to react with 小鼠 due to sequence homology;