WB; Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human TOP2A (isoform 1) has a predicted length of 1,531 residues and MW of 174 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (RKPSTSDDSDSNFEK) corresponding to a region (1466-1480) from human DNA topoisomerase 2-alpha. To enhance the immunological response, this peptide was coupled to carrier protein BSA.