WB; Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human TIMP4 (precursor) has a predicted length of 224 residues and MW of 26 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (YRGHLPLRKEFVDIVQP) corresponding to a region (208-224) from the C-terminus of human Metalloproteinase inhibitor 4. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
来源宿主
兔
反应性
人; 大鼠; expected to react with 小鼠 due to sequence homology;
