WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human PP2A alpha isoform has a predicted length of 309 residues and MW of 36 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (FTKELDQWIEQLNEC) corresponding to a region (6-20) from human PP2A alpha isoform. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
