WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human Sonic Hedgehog protein (precursor) has a predicted length of 462 amino acids and MW of 50 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (RHPKKLTPLAYKQFIPN) corresponding to a region (34-50) from human Sonic Hedgehog protein (SHH). To enhance the immunological response, this peptide was coupled to carrier protein BSA.
