WB; IH, Frozen; Immunohistochemistry (IHC) Western Blotting (WB) and Immunocytochemistry. A concentration of 0.1-0.5 µg/mL is recommended for WB. Human SCG3 (precursor) has a predicted length of 468 residues and MW of 53 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed frozen tissues. Heat mediated antigen retrieval is required. A concentration of 0.5-1.0 µg/mL is recommended for IHC-F. A concentration of 1.0 µg/mL is recommended for Immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (DFINKQADAYVEK) corresponding to a region (438-450) from human Secretogranin-3. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
来源宿主
兔
反应性
人; 大鼠; expected to react with 小鼠 due to sequence homology