WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0 µg/mL is recommended for WB. Human Lamin A has a predicted length of 664 residues and MW of 74 kDa. Human Lamin C has a predicted length of 572 residues and MW of 65 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (FVRLRNKSNEDQSMGNWQ) corresponding to amino acids 451-468 from human Lamin A and Lamin C. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
