WB; Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human HMGB4 has a predicted length of 186 residues and MW of 22 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide RNKFKEQQPNTYVGFKEF which differs by a single residue to a region (24-41) from human High mobility group protein B4 (HMGB4). To enhance the immunological response, this peptide was coupled to carrier protein BSA.