WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. The predicted length of human Smad2 is 467 amino acids and MW of 52 kDa. The predicted length of Smad3 is 425 amino acids and MW of 48 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect Smad 2/3 in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Heat mediated antigen retrieval is required. A concentration of 0.4-1.0 µg/mL is recommended for IHC-F. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (EQNGQEEKWCEKAVKS) corresponding to a region from the N-terminus of human Smad2 and Smad3.
来源宿主
兔
反应性
人; expected to react with 大鼠, 小鼠 due to sequence homology