BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2， such as in the case of translocation of BCL2 to Ig heavy chain locus， is thought to be the cause of follicular lymphoma. Two transcript variants， produced by alternate splicing， differ in their C-terminal ends.
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大肠杆菌中重组表达
溶解建议
Sterile Filtered White lyophilized （freeze-dried） powder. The protein contains 10mM Tris-HCL pH-8， 1mM EDTA and 250mM NaCl. Suspend Bcl-2 in 100?l of 0.5M Acetic acid， over night at 4°C.
Dilute 10 fold into selected buffer system.
BCL-2 has tendency to form intramolecular disulfide bond， 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel， 10mM DTT is recommended.
保存建议
Lyophilized Bcl-2 although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon reconstitution Bcl-2 should be stored at 4℃ between 2-7 days and for future use below
-18℃.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
