LALF has been isolated from the membranes of amebocytes of Limulus polyphemus. LALF is a single-chain polypeptide with a molecular weight of 11,800. The principal structure of the molecule consists of 102 amino acids and is partially homologous with structures of molecules in the lactalbumin-lysozyme family.
LALF has a high affinity binding for LPS and bactericidal for gram negative bacteria. LALF binds the lipid A part of a variety of chemically diverse LPSs.
It can be used for endotoxin removal and detection. LALF reacts with specific antisera in ELISA and Western blots.
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毕赤酵母中重组表达
溶解建议
Sterile Filtered White lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1mg/ml) solution containing no additives. It is recommended to reconstitute the lyophilized LALF in sterile 20mM acetic acid not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
保存建议
Lyophilized LALF although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution LALF should be stored at 4℃ between 2-7 days and for future use below
-18℃.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.