Cytokeratin 14 is a member of the keratin family， the most diverse group of intermediate filaments. Cytokeratin 14 is a type I keratin， is usually found as a heterotetramer with two keratin 5 molecules， a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
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大肠杆菌中重组表达
溶解建议
Sterile Filtered White lyophilized （freeze-dried） powder. The protein （1mg/ml） was lyophilized after from a sterile solution containing 30mM Tris-HCL pH-8， 9.5M urea， 2mM DTT， 2mM EDTA and 10mM methylammonium chloride. It is recommended to reconstitute the lyophilized CK-14 in sterile 18MΩ-cm H2O not less than 100ug/ml， which can then be further diluted to other aqueous solutions.
保存建议
Lyophilized KRT14 although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon reconstitution KRT14 should be stored at 4℃ between 2-7 days and for future use below
-18℃.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
