Keratin 8 and 18 (K8/18) are the major components of intermediate filament (IF) proteins of simple or single-layered epithelia.
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大肠杆菌中重组表达
溶解建议
Sterile Filtered White lyophilized (freeze-dried) powder. The protein (1mg/ml) was lyophilized after from a sterile solution containing 30mM Tris-HCL pH-8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride. It is recommended to reconstitute the lyophilized KRT8 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
保存建议
Lyophilized KRT8 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution KRT8 should be stored at 4℃ between 2-7 days and for future use below
-18℃.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.