Coagulation factor VIII participates in the intrinsic pathway of blood coagulation； factor VIII is a cofactor for factor IXa which， in the presence of Ca+2 and phospholipids， converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein， isoform a， which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein， isoform b， which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A， a common recessive X-linked coagulation disorder.
序列
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来源宿主
CHO中国仓鼠卵巢细胞中重组表达
溶解建议
Sterile Filtered White lyophilized （freeze-dried） powder. The protein was lyophilized from a solution containing 50mM histidine， 0.3M NaCl， 5mM CaCl2， 0.02% Tween 80， 20mM sucrose， pH- 6.8. It is recommended to reconstitute the lyophilized Factor-VIII in 1ml sterile 18MΩ-cm H2O， which can then be further diluted to other aqueous solutions.
保存建议
Lyophilized Factor-VIII although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon reconstitution Factor-VIII should be stored at 4℃ between 2-7 days and for future use below -18℃.
Please prevent freeze-thaw cycles.
