Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is wilyde used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant （Kd） of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution （K_assoc = 10**14）. The complexes are also extremely stable over a wide range of temperature and pH.
序列
请咨询我们客服或者请查看品牌官方网站
来源宿主
细菌中纯化的链霉亲生物素蛋白
溶解建议
Sterile Filtered lyophilized powder. Lyophilized in 10mM potassium phosphate buffer pH 6.5. Gives a clear solution at 5mg/ml in 0.1M NaCl.
保存建议
Streptavidin although stable at 4℃ for 3 weeks， should be stored desiccated below -18℃.
For longer storage in dissolved form add 1mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter.
Please prevent freeze-thaw cycles.
