POU5F1 is a homeodomain transcription factor of the POU family， expressed in embryonic stem （ES） cells and embryonic carcinoma （EC） cells.POU5F1 is significantly involved in the signaling pathway for maintaining self-renewal and pluripotency of ES cells. POU5F1 has 2 distinct DNA binding domains which independently bind half-sites of the canonical octamer motif. This flexibility allows POU5F1 to bind with distinct DNA motifs by forming heterodimers with other transcription factors or by forming homodimers in several conformations. Human POU5F1 contains a 75aa POU specific （POUS） domain and a 60aa POU-Homeo-（POUH） domain connected by a linker region. The Human POU5F1 specifically interacts with Octamer motif ATGCAAAT. In addition， 2 proline-rich domains in the N-terminal and C-terminal regions are vital for POU5F1 transactivation. POU5F1 regulates a number of target genes and has been shown to work jointly with other transcription factors including Sox2 as well as Nanog to sustain stem cell potency and self-renewal.
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来源宿主
大肠杆菌中重组表达
溶解建议
提供的重组人POU class 5 同源框1产品形式为经无菌过滤处理的无色溶液.
保存建议
重组人POU class 5 同源框1产品在4℃保存请2 - 4周内将使用；-20℃可长期保存.产品若长期保存，建议添加载体蛋白（0.1% HSA或BSA）.避免反复冻融.
