Viral MIP-2 cDNA encodes a 94 amino acid residue precursor protein with a 23 aa residue signal peptide that is cleaved to yield a 71 aa residue mature protein. Among human chemokines, vMIP-2 is most closely related to MIP-1α, sharing approximately 41% amino acid sequence identity. At the amino acid sequence level, vMIP-1 and vMIP-2 also share 48% identity. vMIP-1 and vMIP-2 are more closely related to one another phylogenetically than to other human chemokines, suggesting that they may have arisen by gene duplication within the virus rather than by two independent gene aquisitions. vMIP-2 binds to the CCR3 chemokine receptor through which eotaxin and other β chemokines activate eosinophils. vMIP-2 has been shown to activate and chemoattract human eosinphils.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20C. Further dilutions should be made in appropriate buffered solutions.
保存建议
This lyophilized preparation is stable for several weeks at 2-8C, but should be kept at -20C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -70C. Avoid repeated freeze/thaw cycles.
