MIP-3/CCL23 is a CC chemokine that signals through the CCR1 receptor. MIP-3 chemoattracts monocytes, resting T-lymphocytes and neutrophils, but does not chemoattract activated lymphocytes. Additionally, MIP-3 has been shown to inhibit colony formation of bone marrow myeloid immature progenitors. Alternative splicing of the MPIF1 gene results in two mRNAs that encode a short (CKβ8) and a long (CKβ81) isoform of the chemokine. CKβ8 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature CKβ8 (aa 22 120). Additional N terminal processing of the 99 aa residue variant can generate a 75 aa residue CKβ8 (aa 46 120) that is significantly more active than the 99 aa residue variant.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20
