The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific， and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1， which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
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来源宿主
SF9昆虫细胞中重组表达
溶解建议
提供的人AKT1激酶（AKT-1）产品为过滤灭菌的透明溶液；其中0.1mg/ml人AKT1激酶（AKT-1）产品溶解于50mM Tris pH 7.5，0.15M NaCl，0.27M 蔗糖，10mM 巯基乙醇，1mM EGTA. Carrier-free溶液中.
