- 中文名称
Prostaglandin E2 (PGE2) BioAssay ELISA Kit
- 英文名字
- Prostaglandin E2 (PGE2) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- P9053-30
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Prostaglandin E2 (PGE2) is derived from PGH2 which in turn is synthesized from arachidonic acid through the cyclooxygenase pathway. Many cell types, such as epithelial cells, fibroblasts, and macrophages produce PGE2. PGE2 possesses vasoactivity, modulates immune funtions, regulates sleep-awake cycles and exhibits many other activities.Principle Of The Assay:This is an monoclonal ELISA (Enzyme-Linked ImmunoSorbent Assay) for the quantitative analysis of Prostaglandin E2 levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the PGE2 in the sample for a limited number of binding sites on the antibody coated plate. The sample or standard solution is first added to the microplate. Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding sites is taking place. The plate is then washed removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate which generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650nm. The extent of color development is inversely proportional to the amount of PGE2 in the sample or standard. For example, the absence of PGE2 in the sample will result in a bright blue color, whereas the presence of PGE2 will result in decreased or no color development.Kit Components:P9053-30A: EIA Buffer: 30ml. Provided to dilute enzyme conjugate and PGE2 standards.P9053-30B: Wash Buffer (10X): 20ml. Dilute 10 fold with deionized water. Diluted wash buffer is used to wash all unbound enzyme conjugate, samples and standards from the wells after the one hour incubation.P9053-30C: Substrate: 20ml. Stabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus Hydrogen Peroxide (H2O2) in a single bottle. It is used to develop the color in the wells after they have been washed. Light sensitive. Keep substrate refrigerated.P9053-30D: Extraction Buffer (5X): 30ml. Dilute 5 fold with deionized water. This buffer is used for diluting extracted and non-extracted samples.P9053-30E: Prostaglandin E2 Enzyme Conjugate: Two vials of lyophilized PGE2 HRP conjugate. Reconstitution with 75ul of deionized water results in a 50:1 concentrate. Blue capped vials.P9053-30F: Prostaglandin E2 Standard: 100ul. PGE2 standard provided at the concentration of 1ug/ml. Glass vial. Solution is viscous, take precaution when pipetting.P9053-30G: Prostaglandin E2 Antibody Coated Plate: A 96 well Costar microplate precoated with monoclonal mouse antibody against PGE2 The plate is ready for use as is. Do not wash.Storage and Stability:Store all components at 4°C except P9053-30E. Store lyophilized P9053-30E at -20°C. Store reconstituted P9053-30E at 4°C.
- 产品特点
- 针对Prostaglandin E2 (PGE2) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Prostaglandin E2 (PGE2) BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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