- 中文名称
PBEF, Human, BioAssay ELISA Kit (Nicotinamide Phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B Cell Colony-enhancing Factor 1, Pre-B Cell-enhancing Factor, Visfatin, NAMPT, PBEF1)
- 英文名字
- PBEF, Human, BioAssay ELISA Kit (Nicotinamide Phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B Cell Colony-enhancing Factor 1, Pre-B Cell-enhancing Factor, Visfatin, NAMPT, PBEF1)
- 供应商
- USBiological
- 产品货号
- P3213-90
- 产品报价
- ￥1/100Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Nampt, nicotinamide phosphoribosy-ltransferase, is the rate limiting enzyme of the mammalian NAD biosynthesis pathway. The circulating NMN (nicotinamide mononucleotide) and NAD are taken up by beta cells and converted to NAD by Nmnat (nicotinamide mononucleotide adenyltransferase) and intracellular Nampt. The Nampt plays a critical role in enhancing life span and protecting against oxidative damage. The Nampt (Visfatin/PBEF) (human) Intracelluar ELISA Kit is to be used for the in vitro quantitative determination of intracellular mouse or rat Nampt (Visfatin/PBEF). This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG and TMB (3,3',5,5'-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of Nampt in the samples. This ELISA is specific for the measurement of natural and recombinant human Nampt. It does not cross-react with human adiponectin, human resistin, human RELM-b, human leptin, human GPX3, human ANGPTL4, human FABP4, human ANGPTL6, human PAI1. The assay range is 0.063-16ng Nampt/ml and a detection limit of 30pg/ml (based on adding two standard deviations to the mean value of the zero standard).Sample Type:Cell lysatesIntended Use:For the measurement of natural and recombinant human Nampt.Sensitivity:30pg/mlRange:0.063-16ng Visfatin/mlSpecificity:MammalianTest Principle:This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG and TMB (3,3',5,5'-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of Nampt in the samples.Kit Components:Pre-coated Microtiter Plate: 12x8 well stripsWash Buffer (10X): 1x50mlDiluent (5X):1x50mlDetection Antibody: 1x12mlDetector 100X (Hrp conjugated anti-IgG): 1x150ulHuman Nampt Standard (lyophilized, 32ng): 1 vialHuman Nampt QC Sample (lyophilized): 1 vialTMB Substrate Solution: 1x12mlStop Solution: 1x12mlPlate Sealers: 3 eachLysis Buffer (10X): 1x12mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对PBEF, Human, BioAssay ELISA Kit (Nicotinamide Phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B Cell Colony-enhancing Factor 1, Pre-B Cell-enhancing Factor, Visfatin, NAMPT, PBEF1)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到PBEF, Human, BioAssay ELISA Kit (Nicotinamide Phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B Cell Colony-enhancing Factor 1, Pre-B Cell-enhancing Factor, Visfatin, NAMPT, PBEF1)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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