- 中文名称
p-Nitrophenyl Phosphate (pNPP, KR-pT-IRR), Phosphatase Assay Kit, BioAssay
- 英文名字
- p-Nitrophenyl Phosphate (pNPP, KR-pT-IRR), Phosphatase Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- N2600-36
- 产品报价
- ￥23
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Description:Para-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at 405 nm on a spectrophotometer. phosphatase p-Nitrophenyl phosphate p-nitrophenol + phosphateThis homogeneous mix-and-measure assay involves simply adding a single reagent to the phosphatase and measuring the product formation using any absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37°C. In addition, the reagents are compatible with ELISA assays in which alkaline phosphatase conjugated secondary antibody is used. This kit is well adapted for a number of applications. For example, it can be utilized for direct characterization of enzyme activity and for assay condition optimization. It can also be applied for research diagnosis of diseases that are associated with increased levels of alkaline phosphatase. Typical diseases include liver disease, bone disease, Hodgkin's disease, congestive heart failure, Fanconi's syndrome, hyperparathyroidism, intestinal disease and abdominal bacterial infections. Moreover, it can be used to characterize phosphatase inhibitors through high-throughput screening.The kit reagents have been optimized for long shelf life and maximum reproducibility. The reagents are compatible with all liquid handling systems and bulk reagents are available for high-throughput screening of phosphatase inhibitors.Key Features:High sensitivity and wide linear range. The detection limit is generally 3 ng phosphatase or below.Homogeneous and simple procedure. No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes.Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.Applications:Enzyme Activity Assay and Quality Control for phosphatase production. Characterization of Kinetics of phosphatase reaction.Drug Discovery: high-throughput screen for phosphatase inhibitors.Kit Contents:Size (assays)ReagentAssay BufferStop Solution500solid25ml25ml1,000solid50ml50ml2,400solid120ml120mlStorage conditions. The Reagent should be stored in the amber tube at -20°C. The Assay Buffer and Stop Solution are stable at 4°C and room temperature, respectively. Shelf life: 12 months.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.
- 产品特点
- 针对p-Nitrophenyl Phosphate (pNPP, KR-pT-IRR), Phosphatase Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到p-Nitrophenyl Phosphate (pNPP, KR-pT-IRR), Phosphatase Assay Kit, BioAssay产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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