- 中文名称
Mir-10b (MicroRNA 10b) Detection Kit, BioAssay
- 英文名字
- Mir-10b (MicroRNA 10b) Detection Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- M3949-97
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Introduction:MicroRNAs (miRNAs) are endogenous regulators of gene expression that are encoded in the genomes of plants, animals and viruses, then processed into ~19-23 nt, single-stranded molecules that become incorporated into the RNA-induced silencing complex (RISC). RISC mediates down-regulation of gene expression through translational inhibition, transcript cleavage, or both. There is emerging evidence that some microRNAs can function as oncogenes or tumor suppressors. Recent study showed that microRNA-10b (miR-10b) is highly expressed in metastatic breast cancer cells and positively regulates cell migration and invasion. Designed for use with MicroRNA qRT-PCR primer sets, the miR-10b One-Step qRT-PCR Detection Kit provides a one-step, simple, robust, inexpensive assay for quantitative analysis of mir-10b expression from total RNA samples, or small RNAs enriched samples. MicroRNA qRT-PCR primer sets were uniquely designed and optimized for detecting mature miRNA with high specificity and sensitivity at low PCR annealing temperature. The reverse PCR primer is also served as RT primer for reverse transcription. This kit contains an enzyme mix, a 2X-concentrated qRT-PCR reaction mixture and a miRNA primer set that contain all the reagents (except templates) needed for running qRT-PCR assay. The passive reference dye ROX is included in a separate tube to make this kit adaptable for many real-time QPCR platforms. Total RNA from metastatic breast cancer cell line MDA-MB-231 is supplied with the kit as positive control. Features:• Convenient - All reaction components are supplied for quick and easy set up • Save time – One-step qRT-PCR procedure reduces setup time and liquid handling steps • Wide dynamic range: good linearity and excellent PCR efficiency over a 6 logs of dynamic range • High Sensitivity - detect miRNA expression in as low as 2.5pg total RNA. • Flexible – Compatible with most of the real-time PCR instruments.Applications:• Mir-10b detection and quantification • Breast cancer metastasis studiesDescription: Components in this kit are prepared with pure chemicals according to our proprietary technology. Mir-10b One-Step qRT-PCR Detection Kit provides a one-step, simple, robust, inexpensive assay for detection and quantitative analysis of mir-10b expression from total RNA samples, or small RNAs enriched samples. Quality Control 1 kit of this lot has been tested for amplifying miR-10b from total RNA of metastatic breast cancer cell line MDA-MB-231 over a 6 logs of dynamic range using Stratagene's Mx3005P as a real time PCR instrument. Good linearity and great PCR efficiency is observed and consistent with the previous lot. Components: Reagents are sufficient for 200 assays.Item Amount1. MicroRNA 2X qRT-PCR Reaction Mixture, 1.25ml x 22. MicroRNA qRT-PCR Enzyme Mix*, 100ul3. ROX Reference Dye, 100ul4. miR-10b qRT-PCR Primer Set (25X) 200ul5. MDA-MB-231 Total RNA (25ng/ul) 100ul6. Nuclease-Free PCR Grade Water 3ml* microRNA qRT-PCR enzyme mix contains Reverse Transcriptase, RNase Inhibitor, and Hotstart Taq DNA Polymerase. Storage and Stability Upon receipt, store all components at -20°C in a constant temperature freezer. Avoid repeated freeze/thaw cycles. When stored under these conditions this kit is stable for one year after ship date. The dye in MicroRNA 2x qRT-PCR Mixture and the ROX reference dye are light sensitive and should be kept away from light whenever possible.
- 产品特点
- 针对Mir-10b (MicroRNA 10b) Detection Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Mir-10b (MicroRNA 10b) Detection Kit, BioAssay产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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